Cytomegalovirus: An Ultrastructural Study of the Morphogenesis of Nuclear Inclusions in Human Cell Culture
- 1 September 1981
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 56 (1) , 97-104
- https://doi.org/10.1099/0022-1317-56-1-97
Abstract
Summary We investigated the ultrastructural development and maturation of cytomegalovirus (CMV) nuclear inclusions (NIs) in human embryo thyroid cells at 1 to 144 h post-infection. At 5 h, most cells had rounded from an initial fibroblastic appearance and contained early NIs. At 24 h, early NIs were larger and better defined. At 48 h, although early NIs were still present, most cells had larger and presumably more mature NIs. These latter NIs consisted of several subunits, each made up of a fibrillar network enclosing an electron-lucent area which contained coarse and delicate granules. Also, at 48 h, virus particles were first seen in the nucleoplasm. At 72 h, in cells with more developed NIs, virus particles were closely associated with the fibrillar network. Between 96 and 144 h, the NIs reached maximum size and were made up of numerous subunits. The results indicate that two types of NIs coexist during CMV infection. The appearance of the early and late NIs coincides with the reported peaks of CMV DNA synthesis and thus may explain the biphasic pattern of DNA synthesis in CMV infection. Morphogenetic features of the NIs conform with the hypothesis that synthesis of CMV DNA may occur in the centre of each NI subunit and that the fibrillar network represents condensing capsid proteins.Keywords
This publication has 17 references indexed in Scilit:
- Evidence for Early Nuclear Antigens in Cytomegalovirus-Infected CellsJournal of General Virology, 1976
- CYTOMEGALIC INCLUSION DISEASE - AN ELECTRON MICROSCOPIC HISTOCHEMICAL STUDY OF VIRUS AT NECROPSY1966
- HUMAN CYTOMEGALOVIRUS INFECTION - ELECTRON MICROSCOPIC AND HISTOCHEMICAL CHANGES IN CULTURES OF HUMAN FIBROBLASTS1965
- Human cytomegalovirus. DNA synthesis and migration in infected cells studied autoradiographicallyVirology, 1964
- The Immunofluorescent Focus Technique in Studying the Replication of CytomegalovirusThe Journal of Immunology, 1963
- Human cytomegalovirus. Cytochemical observations of intracellular lesion development correlated with viral synthesis and releaseVirology, 1963
- ELECTRON MICROSCOPY OF SALIVARY GLAND VIRUSESThe Journal of Experimental Medicine, 1958
- Isolation of Intranuclear Inclusion Producing Agents from Infants with Illnesses Resembling Cytomegalic Inclusion Disease.Experimental Biology and Medicine, 1957
- Propagation in Tissue Cultures of a Cytopathogenic Virus from Human Salivary Gland Virus (SGV) Disease.Experimental Biology and Medicine, 1956
- CYTOPATHOGENIC AGENT RESEMBLING HUMAN SALIVARY GLAND VIRUS RECOVERED FROM TISSUE CULTURES OF HUMAN ADENOIDS1956