Propagation in Tissue Cultures of a Cytopathogenic Virus from Human Salivary Gland Virus (SGV) Disease.

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Abstract
Cyto-pathogenic viruses were isolated from the tissues of 2 infants; the first from the salivary gland of an infant in whom infection of the salivary glands with the salivary gland virus (SGV) was established by microscopic examination and the second from the kidney of an infant dying of generalized SGV infection (cyto- megalic inclusion disease). The 2 cytopathogenic agents, producing morphologically identical cellular changes, were propagated serially in cultures of fibroblasts derived from human myometrium. The serial passages of the virus are effected by transfer of the supranatant fluid medium from infected cultures at intervals of 2 to 3 weeks. The cytologic changes are at first focal and develop slowly. The affected cells in the focal lesions are greatly enlarged and contain large intranuclear inclusions. Necrosis with conspicuous dense granules occurs in the centers of the focal lesions and the cytologic changes gradually spread to involve the entire culture. The virus does not grow in cultures of mouse embryonic tissue and is not pathogenic for mice, rabbits or embryonated hen''s eggs.