Requirement for two DNA polymerases in the replication of simian virus 40 DNA in vitro.
- 1 December 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (24) , 9742-9746
- https://doi.org/10.1073/pnas.86.24.9742
Abstract
DNA polymerase .alpha.-primase has long been considered the primary, if not sole, replicative DNA polymerase in eukaryotic cells. However, recent experimetns have provided indirect evidence that a second DNA polymerase may play a role in DNA replication. To identify cellular proteins necessary for DNA synthesis in mammalian cells, we have been studying the cell-free system developed for the replication of simian virus 40 DNA. In this report, we present direct evidence that a second DNA polymerase is required in addition to DNA polymerase .alpha.-primase complex to obtain efficient replication of simian virus 40 origin-containing DNA. This DNA polymerase activity is not affected by monoclonal antibodies that inhibit the activity of DNA polymerase .alpha. and is relatively resistant to the inhibitor [N2-(p-n-butylphenyl)-9-(2-deoxy-.beta.-D-ribofuranosly)guanine 5''-triphosphate]. Moreover, the activity of the polymerase is highly dependent upon the accessory protein, proliferating-cell nuclear antigen. These characteristics are consistent with the hypothesis that this second DNA polymerase is DNA polymerase .delta.This publication has 34 references indexed in Scilit:
- DNA polymerase III, a second essential DNA polymerase, is encoded by the S. cerevisiae CDC2 geneCell, 1989
- Mammalian DNA polymerases .alpha. and .delta.: current status in DNA replicationBiochemistry, 1988
- Coordinated leading and lagging strand synthesis during SV40 DNA replication in vitro requires PCNACell, 1988
- Identification of DNA polymerase .delta. in CV-1 cells: studies implicating both DNA polymerase .delta. and DNA polymerase .alpha. in DNA replicationBiochemistry, 1987
- The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitroNature, 1987
- Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary proteinNature, 1987
- Cyclin/PCNA is the auxiliary protein of DNA polymerase-δNature, 1987
- Human placental DNA polymerase .delta.: identification of a 170 kilodalton polypeptide by activity staining and immunoblottingBiochemistry, 1987
- Properties of two forms of DNA polymerase .delta. from calf thymusBiochemistry, 1986
- A new mammalian DNA polymerase with 3' to 5' exonuclease activity: DNA polymerase δBiochemistry, 1976