Isolation and primary structure of human PHI (peptide HI)
- 3 September 1984
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 174 (2) , 258-261
- https://doi.org/10.1016/0014-5793(84)81169-2
Abstract
The isolation of the human form of PHI (peptide HI) is described. The peptide was purified from human colonic extracts by using a chemical method for the detection of its C-terminal amidated structure. Human PHI consists of 27 amino acid residues and the complete amino acid sequence is: His-Ala-Asp-Gly-Val-Phe-Thr-Ser-Asp-Phe-Ser-Lys-Leu-Leu-Gly-Gln-Leu-Ser-Ala-Lys-Lys-Tyr-Leu-Glu-Ser-Leu-Met-NH2. The differences between the structures of porcine and human PHI are at position 12 (Arg/Lys replacement) and at position 27 (Ile/Met).Human PHIPorcine PHIBovine PHIBrain peptideGut peptideC-terminal amideVIP/PHI precursoKeywords
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