High-throughput genome sequencing of two Listeria monocytogenes clinical isolates during a large foodborne outbreak
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Open Access
- 1 January 2010
- journal article
- Published by Springer Nature in BMC Genomics
- Vol. 11 (1) , 120
- https://doi.org/10.1186/1471-2164-11-120
Abstract
A large, multi-province outbreak of listeriosis associated with ready-to-eat meat products contaminated with Listeria monocytogenes serotype 1/2a occurred in Canada in 2008. Subtyping of outbreak-associated isolates using pulsed-field gel electrophoresis (PFGE) revealed two similar but distinct AscI PFGE patterns. High-throughput pyrosequencing of two L. monocytogenes isolates was used to rapidly provide the genome sequence of the primary outbreak strain and to investigate the extent of genetic diversity associated with a change of a single restriction enzyme fragment during PFGE.Keywords
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