THE ROLE OF RABBIT IA MOLECULES IN IMMUNE FUNCTIONS AS DETERMINED WITH THE USE OF AN ANTI-IA MONOCLONAL-ANTIBODY

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 51  (1) , 35-43
Abstract
A mouse anti-rabbit Ia monoclonal antibody (MAb) was produced that detects an isotypic determinant on all rabbit Ia molecules. This MAb precipitates 3 polypeptide chains with MW of 28,000, 31,000 and 35,000, corresponding to the Ia .beta., Ii and .alpha. chains, respectively. The anti-Ia MAb inhibits the mixed lymphocyte culture by 80%. In secondary in vitro immune response cultures, the anti-Ia MAb inhibits the proliferative response to bovine insulin and poly (Glu50Tyr50). In studies on mitogenesis, the anti-Ia MAb inhibited the response to LPS [lipopolysaccharide] but not to concanavalin A or phytohemagglutinin. The effect of the anti-Ia MAb on other mitogens varies from rabbit to rabbit.