Histochemical demonstration of thiols and disulfides by the fluorescent labeling agent, monobromobimane: An application to the hypothalamo-neurohypophysial system
- 1 January 1980
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 68 (3) , 309-315
- https://doi.org/10.1007/bf00493259
Abstract
Summary The use of the new fluorescent labeling agent monobrombimane for the histological visualization of thiols, and, after reduction, of disulfides, is described. Fluorescent labeling of the disulfide-rich neurosecretory proteins in the hypothalamo-neurohypophysial system of rat and frog is shown. The reaction of the non-fluorescent reagent with thiols results in highly fluorescent materials which resist fading. The labeling procedure is very simple, rapid, and is carried out in one step. It is non-destructive and can allow for biochemical analyses of the labeled tissue.Keywords
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