Complexing of Calcium by Hexametaphosphate, Oxalate, Citrate, and Ethylenediamine-Tetraacetate in Milk. II. Dialysis of Milk Containing Complexing Agents

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Abstract
The influence of hexametaphosphate (HMP), oxalate, citrate and EDTA on salt equilibria in milk was studied by continuous and equilibrium dialyses of milk containing up to 30 m[image] of these agents. Alteration of milk by these agents was assessed by determining the rate of diffusion of Ca and P from milk samples containing various concentrations of the agents. Added sodium-citrate solubilized a portion of the Ca and all of the inorganic phosphate. Oxalate and EDTA made all of the Ca in milk diffusible. The HMP was unique in its action. Both P and Ca were less diffusible when small amounts of HMP were present. Excessive addition induces decomposition of the colloidal inorganic salts similar to that which occurs from citrate. A certain amount of HMP still remained in the nondiffusible phase after dialysis, indicating that HMP combined with the casein complex.