Studies on ribonucleases in Takadiastase
- 1 January 1959
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 46 (1) , 31-37
- https://doi.org/10.1093/jb/46.1.31
Abstract
This enzyme specifically hydrolyzes the secondary phosphate ester bonds of guanosine-3[image]-phosphate (G-3[image]-P) of yeast ribonucleic acid (RNA). The reaction proceeds in 2 stages: (1) transphosphorylative cleavage to form guanosine-2[image],3[image]-cyclic phosphate (G-2[image],3[image]-P) or G-2[image],3[image]-P-termlnal oligonucleo-tide(s); (2) hydrolysis to free guanosine-3[image]-phosphate(G-3[image]-P) or G-3[image]-P-terminal oligonucleotide(s), respectively by use of phosphomonoesterase the freed mononucleotide, namely G-3[image]-P, is determined as 40% of total G-3[image]-P residues in RNA. This finding indicates that there are a considerable number of two or more adjacent G-3''-P residues in the RNA molecule.This publication has 8 references indexed in Scilit:
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