Saliva Incubated with Glucose as a Replacement for Tryptophane in a Synthetic Medium Supporting the Production of Acid by Lactobacillus Acidophilus

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Abstract
When human saliva is incubated with glucose for 24 hrs. or more it can be substituted for tryptophane in a synthetic medium used to promote the growth of an oral strain of Lactobacillus. An aliquot of the same pooled saliva, when incubated without glucose, does not support maximum growth of the test organism. When saliva incubated with glucose is used as a substitute for casein hydrolyzate in the synthetic medium, growth of the test organism occurred, but was only about 1/s that obtained when casein hydrolyzate was used. This suggests that glucose added to incubating saliva makes available, possibly through the mechanism of enhancement of proteolysis, some of the amino acids found necessary for the growth of the test organism.