Activity of Pentose Cycle Dehydrogenase Systems in Liver and in Spleen of Rats after Whole-Body X-Irradiation

Publisher Website
Google Scholar
Abstract
The dehydrogenase system activity of homo-genates of liver and of spleen was measured microchemically in 2 components of the pentose cycle (glucose-6-phosphate-dependent and 6-phosphogluconate-dependent) by the use of a tetrazolium salt (INT). Assays of liver and of spleen homogenates were done at successive time periods after 625 r WBR of young adult male Charles River rats. The fall in the dehydrogenase system activity for both substrates and both organs was biphasic. The depression in activity was evident immediately after irradiation. The spleen showed a greater effect than did the liver at that time.