The Characterization of Shiga Toxin‐Non‐Producing Escherichia coli Serotype O157:H7 Isolated from Carcasses of Cattle at a Slaughter House

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Abstract
A bacteriological investigation of Shiga toxin (Stx)‐producing Escherichia coli (STEC) 0157:H7 was performed on 298 carcasses of cattle at slaughter houses between July 1996 and January 1997 in Gifu Prefecture, Japan. As a result, four Stx‐non‐producing Escherichia coli O157:H7 strains were isolated from two slaughtered carcasses of cattle. The purpose of this study was to examine the characterization of isolates. Isolates possessed the E. coli attaching and effacing gene (eaeA), and hemolysin gene (hlyA), and harbored 3.0‐MDa and 60‐MDa plasmids. The Xba I pulsed‐field gel electrophoresis (PFGE) pattern showed three similar patterns. Consequently, a closely related genotype of Stx‐non‐producing E. coli O157:H7 may widely exist in cattle.