β tubulin of bull sperm is polyglycylated
Open Access
- 8 May 1995
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 364 (2) , 147-151
- https://doi.org/10.1016/0014-5793(95)00373-h
Abstract
Car☐y‐terminal fragments of α and β tubulin from bull sperm were isolated and characterized by automated sequencing and mass spectrometry. About 60% of sperm α tubulin is polyglycylated. The lateral chain, which can reach 13 residues in length, is covalently attached via an isopeptide bond. The fully detyrosinated sperm α tubulin lacks polyglycylation. Thus mammalian sperm microtubules differ from the ciliary axonemal microtubules of the protozoanParamecium for which others have documented a complete polyglycylation of both α and β tubulin.Keywords
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