Survival and biochemical characteristics of stored red cells preserved with citrate-phosphate-dextrose-adenine-one and two and prepared from whole blood maintained at 20 to 24 degrees C for eight hours following phlebotomy
- 1 March 1984
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 24 (2) , 115-119
- https://doi.org/10.1046/j.1537-2995.1984.24284173340.x
Abstract
Studies were conducted to evaluate the characteristics of red cells stored for 35 days following preparation from units of whole blood anticoagulated with citrate-phosphate-dextrose-adenine-1 and 2 (CPDA-1 and CPDA-2) and maintained at 20-24.degree. C for 8 h after phlebotomy. The mean (.+-. 1 SD) 24-h survival for transfused CPDA-1 autologous red cells with hematocrit levels of 78.1 .+-. 2.3% was 78.0 .+-. 8.1% (n = 9). The 24-h survival of red cells from units preserved with CPDA-2 with hematocrit levels of 79.3 .+-. 4.5% was 74.8 .+-. 8.6% (n = 15). This difference in survival was not significant. Red cells from control units stored for 1 day showed a 24-h survival of 91.1 .+-. 4.2% (n = 7). During the 8-h holding, red cell ATP levels increased by 15-25% in units drawn into both CPDA-1 and CPDA-2. After the initial 8-h period, the red cell 2,3 diphosphoglycerate levels were 54 .+-. 12% (mean .+-. 1 SD) of initial levels in units drawn into CPDA-1 and 58 .+-. 8% of initial levels in units drawn into CPDA-2. Following 35 days of storage, units of red cells prepared from whole blood drawn into CPDa-1 and CPDA-2 had comparable plasma cation and NH3 levels and similar amounts of cell-free Hb. Red cells can be stored satisfactorily for 35 days when prepared from whole blood held at 20-24.degree. C for 8 h.Keywords
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