Isolation and characterization of the centromere from chromosome V (CEN5) of Saccharomyces cerevisiae.
Open Access
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 4 (1) , 86-91
- https://doi.org/10.1128/mcb.4.1.86
Abstract
We have cloned a functional centromeric DNA sequence from Saccharomyces cerevisiae. Using the 2 mu chromosome-loss mapping technique and meiotic tetrad analysis, we have identified this DNA sequence as the centromere of chromosome V (CEN5). The CEN5 sequence has been localized on an 1,100-base-pair BamHI-BglII restriction fragment. Plasmids containing CEN5 and an autonomously replicating sequence are mitotically stable in S. cerevisiae and segregate in a Mendelian fashion during meiosis.This publication has 26 references indexed in Scilit:
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