A fourth type of rabbit protein kinase C

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Abstract
Three rabbit cDNA clones coding for three types of protein kinase C (PKC .alpha., .beta., and .gamma.) have recently been identified and the structures determined [Ohno, S., Kawasaki, H., Imajoh, S., Suzuki, K., Inagaki, M., Yokokura, H., Sakoh, T., and Hidaka, H. (1987) Nature (London) 325, 161-166]. By use of these cloned cDNAs as hybridization probes, a fourth type (.delta.) of cDNA clone, which encodes a protein highly homologous to PKC .alpha.,.beta. and .gamma., was identified. PKC .delta. is composed of 697 amino acid residues and contains several peptide sequences determined at the protein level with the brain PKC preparation. This indicates that this molecular type (PKC .delta.) is, along with PKC .alpha.,.beta. and .gamma., a constituent of the brain PKC preparation. Sequence comparison among the four PKC types revealed that PKC .delta. is somewhat distinct from the other PKC types. PKC .delta. shows 99% amino acid sequence identity with rat PKC type I [Knopf, J.L., Lee, M.-H., Sultzman, L.A., Kriz, R.W., Loomis, C.R., Hewick, R.M. and Bell, R.M. (1986) Cell (Cambridge, Mass.) 46, 491-502], indicating relationship of these PKC types. The mRNA for PKC .delta. is exclusively concentrated in the brain.