The 5′ end group of tobacco mosaic virus RNA is m7G5′ppp5′Gp
Open Access
- 1 January 1975
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (7) , 1189-1202
- https://doi.org/10.1093/nar/2.7.1189
Abstract
RNA extracted from CsCl.purified virions of tobacco mosaic virus is shown to give rise to an unusual nucleotide on digestion with RNAase T2, in addition to the four major nucleotides. This minor component has the electrophoretic characteristics of a phosphorylated end group, but is partially resistant to bacterial alkaline phosphatase. It is, however, a substrate for venom phos-phodiesterase or nucleotide pyrophosphatase, yielding products which imply the structure m7G5′ppp5′Gp. TMV RNA, like many animal cellular and viral mRNAs recently examined, therefore has a 5′ terminus blocked by a methylated nucleotide inverted with respect to the rest of the chain.Keywords
This publication has 32 references indexed in Scilit:
- Modified nucleosides and bizarre 5′-termini in mouse myeloma mRNANature, 1975
- 5′-Terminal 7-methylguanosine in eukaryotic mRNA is required for translationNature, 1975
- Methylated nucleotides block 5'-terminus of vaccinia virus messenger RNA.Proceedings of the National Academy of Sciences, 1975
- Sequences containing methylated nucleotides at the 5′ termini of messenger RNAs: Possible implications for processingPublished by Elsevier ,1974
- Subunit complex III-IV as the nucleotide binding site of Qβ replicaseNature, 1974
- TMV-RNA is not methylated and does not contain a polyadenylic acid sequenceVirology, 1973
- Bacteriophage Qβ Replicase Contains the Protein Biosynthesis Elongation Factors EF Tu and EF TsProceedings of the National Academy of Sciences, 1972
- Polar reconstitution of tobacco mosaic virus (TMV)Virology, 1971
- Studies of Nucleotide Sequences in Tobacco Mosaic Virus Ribonucleic Acid. III. Periodate Oxidation and Semicarbazone Formation*Biochemistry, 1966
- THE PURINE AND PYRIMIDINE COMPOSITION OF THE TOBACCO MOSAIC VIRUS AND THE HOLMES MASKED STRAINJournal of Biological Chemistry, 1954