Expression of Differentiated Functions in Hepatoma Cell Hybrids: High Frequency of Induction of Mouse Albumin Production in Rat Hepatoma-Mouse Lymphoblast Hybrids
- 1 March 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (3) , 927-931
- https://doi.org/10.1073/pnas.71.3.927
Abstract
We have studied the production of serum albumin by somatic hybrids between well-differentiated 2 s and 1 s rat hepatoma cells (Faza), which produce serum albumin, and sub-diploid mouse leukemic lymphoblasts (Lc), which do not produce albumin. We determined the rat or mouse origin of the albumin by double immunodiffusion, using immuno-adsorbed noncrossreacting antisera. Each of 12 karyologically identified 2 s hybrid clones (Lc2F) produces both rat and mouse albumin. Moreover, unlike 1 s hybrids reported previously, eight of nine 1 s hybrids (LcF) also produce mouse albumin; six of them produce rat albumin as well. One clone from the 1 s cross produces only rat albumin.Keywords
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