Photodynamic Activity of Acridine Orange: Peroxide Radical Induction in DNA and Synthetic Polynucleotides
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 45 (5) , 487-495
- https://doi.org/10.1080/09553008414550691
Abstract
As shown by electron paramagnetic resonance, acridine orange induces the formation of peroxide radicals in DNA when dye-DNA mixtures frozen at 77 K are irradiated with visible light. The reaction is oxygen dependent and strongly reduced by the addition of an electron scavenger. Factors of the medium can modulate the reaction: an ionic strength increased up to 0·3 greatly enhances the dye efficiency whereas the presence of phosphate ions has an inhibiting influence. Acridine orange, which is slightly less efficient than proflavine on native DNA, induces an important peroxide radical formation in poly(dG).poly(dC) but has no action on the poly(dA).poly(dT)polymer.Keywords
This publication has 25 references indexed in Scilit:
- MECHANISMS FOR DYE‐MEDIATED PHOTODYNAMIC ACTION: SINGLET OXYGEN PRODUCTION, DEOXYGUANOSINE OXIDATION AND PHAGE INACTIVATING EFFICIENCIESPhotochemistry and Photobiology, 1982
- DYE BINDING AND PHOTODYNAMIC ACTIONPhotochemistry and Photobiology, 1981
- ANALYSIS OF THE MECHANISM OF PHOTODYNAMIC INDUCTION OF SYNTHESIS OF A POLYPEPTIDE IN ARTHROBACTER SP.Photochemistry and Photobiology, 1980
- CELLULAR AND SUBCELLULAR MECHANISMS OF PHOTODYNAMIC ACTION: THE 1O2 HYPOTHESIS AS A DRIVING FORCE IN RECENT RESEARCHPhotochemistry and Photobiology, 1978
- A SURVEY OF IN VIVO PHOTODYNAMIC ACTIVITY OF XANTHENES, THIAZINES, AND ACRIDINES IN YEAST CELLSPhotochemistry and Photobiology, 1977
- PHOTOSENSITIZING EFFICIENCY OF PROFLAVINE BOUND TO POLYADENYLIC ACID AS STUDIED BY EPRPhotochemistry and Photobiology, 1976
- LIGHT‐INDUCED FREE RADICALS IN ORIENTED DNA‐PROFLAVINE COMPLEXESPhotochemistry and Photobiology, 1975
- INDUCTION OF FREE RADICALS IN DNA BY PROFLAVINE AND VISIBLE LIGHT: INFLUENCE OF OXYGEN AND IONIC STRENGTHPhotochemistry and Photobiology, 1974
- Study of the interaction of DNA and acridine orange by various optical methodsBiopolymers, 1972
- Conversion of Solvated Electrons into Hydrogen Atoms in the Photo- and Radiation Chemistry of Aqueous SolutionsThe Journal of Chemical Physics, 1962