Competition between cellular and viral mRNAs in vitro is regulated by a messenger discriminatory initiation factor.
- 1 September 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (9) , 3040-3044
- https://doi.org/10.1073/pnas.73.9.3040
Abstract
Encephalomyocarditis viral RNA has previously been shown to outcompete host cellular mRNA has for translation in vitro in crude and fractionated protein synthesizing systems. In the present communication it is shown that the competition is regulated by an initiation factor or complex of factors, and not the 40S initiation complex per se. The factor primarily involved is the murine equivalent of a component present in a partially purified preparation of rabbit initiation factor M3. Both the murine and rabbit factors are clearly messenger discriminatory.Keywords
This publication has 10 references indexed in Scilit:
- A protein binding the methylated 5'-terminal sequence, m7GpppN, of eukaryotic messenger RNA.Proceedings of the National Academy of Sciences, 1976
- Evidence for role of m7G5′-phosphate group in recognition of eukaryotic mRNA by initiation factor IF-M3Nature, 1976
- The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.Proceedings of the National Academy of Sciences, 1976
- 5'-terminal structure of poliovirus polyribosomal RNA is pUp.Proceedings of the National Academy of Sciences, 1976
- Nonribosomal proteins associated with eukaryotic native small ribosomal subunits.Proceedings of the National Academy of Sciences, 1975
- Model for the regulation of mRNA translation applied to haemoglobin synthesisNature, 1974
- Discrimination between Messenger Ribonucleic Acids by a Mammalian Translation Initiation FactorProceedings of the National Academy of Sciences, 1973
- Specificity in Initiation of Protein Synthesis in a Fractionated Mammalian Cell-free SystemNature New Biology, 1973
- Initiation of Eukaryotic Protein Synthesis: [Met-tRNAf.40S Ribosome] Initiation Complex Catalysed by Purified Initiation Factors in the Absence of mRNANature New Biology, 1973