Some physicochemical properties of human fibrinogen

Abstract
On prolonged electrophoretic migration, purified human fibrinogen showed complex behavior. Slight heterogeneity was demonstrated by isoelectric boundary spreading. Ultracentrifugal examination of human fibrinogen was made under various conditions of protein concentration, pH and ionic strength. The fall of the sedimentation coefficient at low concentrations suggests a disaggregation in rapid equilibrium. Some heterogeneity was shown by boundary analysis. The diffusion coefficient of fibrinogen was determined. Its increase at low concentrations suggests disaggregation. The diffusion boundaries deviate from the ideal form. The molecular weight of the undissociated molecule was 341,000110,000. Bovine fibrinogen showed a difference in variation of the sedimentation coefficient with concentration from that of human fibrinogen, although the sedimentation coefficients at infinite dilution were the same.
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