EFFECT OF PYRAN CO-POLYMER ON ACTIVATION OF MURINE MACROPHAGES - EVIDENCE FOR INCOMPLETE ACTIVATION BY USE OF FUNCTIONAL MARKERS

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 43  (8) , 3633-3637
Abstract
The degree of activation [for tumor cytotoxicity] of peritoneal macrophages elicited by pyran copolymer (MVE-2) was studied in C57BL/6J mice. When cytotoxicity was examined under endotoxin-free culture conditions, the pyran-elicited macrophages could not complete cytolysis of tumor target cells. The macrophages completed cytolysis when pulsed with endotoxin [from Escherichia coli]. These results were obtained when either the interval between injection of the pyran copolymer and harvest of the macrophage or the dose of pyran was varied. The pyran-elicited macrophages expressed 5 markers considered to be typical of inflammatory macrophages and bound tumor cells to an augmented degree. The pyran-elicited macrophages were capable of secreting a potent cytolytic proteinase when pulsed with endotoxin, but did not secrete cytolytic proteinase spontaneously. The pyran-elicited macrophages, in contrast to inflammatory macrophages, could effect cytostasis of tumor cells; their cytostatic potential was also augmented by addition of endotoxin. Pyran copolymer apparently elicits primed, but not fully activated murine macrophages.