Determination, Recovery, Identification and Renal Clearance of Conjugated Adrenal Corticoids in Human Peripheral Blood.

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Abstract
Following ethanol precipitation of human plasma the free corticoids were extracted with methylene chloride as were the conjugated corticoids after hydrolysis with 300-500 units per ml of beta-glucu-ronidase. Recoveries of tetrahydro-E monoglucuronide added to plasma were quantitatively recovered by this method. Acid hydrolysis was unsatisfactory. After giving large doses of free tetrahydro-E orally to a human subject, this steroid was recovered from plasma both in the free and conjugated fractions, the latter case in amounts exceeding 30 mg/100 ml of plasma. Clearance studies demonstrated that the free steroid was excreted in the urine at an average rate of 11.2% and the conjugated 71.0% as compared with creatinine.

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