Effects of Age and Sex on the Electrophoretic Fractions of the Serum Glycoprotein

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Abstract
Specimens of serum were collected from 198 clinically healthy persons, chosen to provide approximately 10 males and 10 females in each decade up to the tenth. After electrophoretic separation using the Spinco method B, the strips were stained by the periodic acid-Schiff reaction according to Köiw and Grönwall (Scand. J. Clin. Lab. Invest. 4, 244, 1952) and scanned on the Analytrol integrating densitometer at 550 mµ. Total glycoprotein was estimated on an ethanol precipitate by the tryptophan-borosulfuric acid reaction (Badin, Jackson, and Schubert, Proc. Soc. Exptl. Biol. Med. 84, 288, 1953). The analytical error and normal values for the method were estimated; those for males did not differ from those for females. The means and standard deviations for groups showing statistically significant differences (P 1, 12.5 ± 4.3; alpha2, under 60 years, 47.4 ± 12.1; alpha2, over 60 years,59.1 ± 16.0; beta, under 10 years, 27.2 ± 6.6; beta, 10 to 90 years, 38.4 ± 8.2; beta, over90 years,54.0 ± 17.7; gamma,26.4 ± 7.1. The results were compared with those obtained by other published procedures.