Radioimmunoassay of Digoxigenin Glycosides in Digitalis lanata

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Abstract
Radioimmunoassay (RIA) was used in the analysis of digoxigenin glycoside contents of crude ethanolic extracts prepared from fresh leaf samples of D. lanata. The commercial antidigoxin [animal] antiserum used in the assay was highly specific against the digoxigenin aglycone. Steroid derivatives with only slight structural differences from digoxigenin remained practically unassayed. The method is highly sensitive and excellent values for accuracy, precision and correlation were obtained. Digoxigenin glycoside levels of 2nd yr D. lanata leaves were higher than those of 1st yr leaves.