CD 4-/CD 8- T cells: amplification in spleens of mice following in vivo treatment with monoclonal antibody anti-L3T4

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Abstract
Amplification of L3T4‐/Ly‐2‐(CD 4‐/CD 8‐) T cells following in vivo administration of anti‐L3T4 monoclonal antibody was examined in the spleen of mice by flow cytometry and immunohistochemistry. BALB/c mice were given 4–7 weekly injections of either anti‐L3T4 (1 mg/week) or phosphate‐buffered saline (control group), and dispersed spleen cells and tissue sections analyzed for the presence of Thy‐1.2+, L3T4+, or Ly‐2+ cells, and for the absence of both L3T4 and Ly‐2 on Thy‐1.2+ cells. Prior to treatment, L3T4+ and Ly‐2+ cells accounted for virtually all Thy‐1.2+ cells in approximately a 2:1 ratio. Following anti‐L3T4 treatment, L3T4+ cells were depleted, and Ly‐2+ cells accounted for about 213 of the Thy‐1.2+ cells. A population of L3T4‐Ly‐2 ‐ T cells was generated that accounted for 20‐30% of the Thy‐1.2+ cells, accounted for most of the Thy‐1.2+ cells in red pulp, and was also present among the predominant Ly‐2+ cells in periarteriolar sheaths.