High-efficiency liquid-scintillation counting of 14C-labelled material in aqueous solution and determination of specific activity of labelled proteins

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Abstract
Inexpensive scintillation mixtures are described which enable the detection of as little as 40[mu][mu]c of 14c in aqueous solution with an efficiency of counting of over 80%. A rapid method for the counting of alkaline, acidic and neutral aqueous solution of up to 1ml. volume is described. Ethanol or 2-ethoxyethanol is used as blending agent. The scintillation counting of alkaline solutions is applied to the accurate determination of the specific activity of 14c-labelled proteins from plant tissues. Attention has been paid to the importance of a standardized washing procedure for the removal of all traces of radioactive material from glassware.