Calcification XIX. Calcification of Transplanted Rachitic Bone.

Publisher Website
Google Scholar
Abstract
Calcification of the rachitic metaphysis, with typical silver line test, was obtained in tibial slices that were transplanted to rats on a normal diet. Similar transplants to rats on a rachitogenic diet did not calcify. This difference was probably due to the higher Ca x P product in the body fluids of animals on a normal diet as compared to the product of animals on a rachito-genic diet. In vitro calcification was obtained in the sera of normal animals, in the sera of rachitic animals to which phosphate was added to raise the Ca x P product but not in the unmodified sera of rachitic animals. In vitro calcification was obtained in inorganic solutions with a Ca x P product of 50 but not with a Ca x P product of 20. Transplanted deep freeze stored bone slices treated with CaCl2 prior to deep freeze calcified more intensively than deep freeze stored bones not treated with Ca, as measured by the silver line test. Calcification in vitro studies indicated reversible inactivation of calcifiability of stored frozen bone which could be prevented or restored by CaCl2 treatment. It is concluded that by standardizing both the rachitic sections and the host animal, a transplantation technique would permit the study of calcification after experimental modification of either the rachitic section or the composition of body fluid in the host animal.