Pulsed laser fluorophore deposition: a method for measuring the axial resolution in two-photon fluorescence microscopy
Open Access
- 1 September 1995
- journal article
- Published by Optica Publishing Group in Journal of the Optical Society of America A
- Vol. 12 (9) , 2072-2076
- https://doi.org/10.1364/josaa.12.002072
Abstract
We report the generation of thin (< 50 nm) fluorescence layers on a glass substrate in the focal region of a highnumerical- aperture lens by employing subpicosecond pulses of 0.75-TW/cm2 peak intensity. The conditions for generating the fluorescence layers are described. We find that the fluorescence molecules at the glass interface are less affected by bleaching than those in the surrounding area. The fluorescence layers are suited for measuring and monitoring the axial resolution of two-photon fluorescence microscopes.Keywords
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