Cloning, functional expression and mRNA distribution of an inwardly rectifying potassium channel protein
- 26 June 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 367 (2) , 127-131
- https://doi.org/10.1016/0014-5793(95)00527-g
Abstract
In GH3/B6 cells at least two different inward K+ currents are observed that are regulated by thyrotropin-releasing hormone and somatostatin, respectively. Using a polymerase chain reaction based approach a cDNA was isolated and functionally expressed in human embryonic kidney cells that encodes an inward rectifier K+ channel, rIRK3, with a predicted molecular mass of 49.7 kDa. Corresponding transcripts of 2.6 kb have been detected in rat brain, pituitary and GH3/B6 cells. In situ hybridization revealed that rIRK3 mRNA is distributed throughout the brain and occurs predominantly in the piriform cortex, indusium griseum, supraoptic nucleus, facial nucleus and cerebellar Purkinje cells.Keywords
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