DNA synthesis during germination of Bacillus subtilis spores.

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Abstract
The effect of chloramphenicol on DNA synthesis during germination of B. subtilis spores was studied. Analysis of DNA synthesized after the addition of chloramphenicol at various times showed that a protein is synthesized before each cycle of generation in order to initiate chromosome replication. Repair-type synthesis was found in spores germinating in the presence of chloramphenicol and under these conditions there was no indication of normal replication. The presence of DNA polymerase activity was demonstrated in the spore. The nature of this DNA polymerase was discussed in relation to the initiation mechanism of chromosome replication.