LABELING PROCEDURES EMPLOYING CRYSTALLINE FLUORESCEIN ISOTHIOCYANATE
- 1 October 1961
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 82 (4) , 534-+
- https://doi.org/10.1128/jb.82.4.534-537.1961
Abstract
Griffin, CharlesW. (Baltimore Biological Laboratory, Baltimore, Md.),Theodore R. Carski, and George S. Warner. Labeling procedures employing crystalline fluorescein isothiocyanate. J. Bacteriol.82:534–537. 1961.—The effect of altering the ratio of dye to protein in the labeling of three immune globulins with crystalline fluorescein isothiocyanate was the purpose of the study. Direct staining of group A streptococci and rabies-infected mouse brain, and indirect staining ofTreponema pallidum(Nichols strain) comprised the three immunological systems selected for investigation. Maximal specific staining and minimal nonspecific background staining were demonstrated by the use of low dye-to-protein labeling ratios.Keywords
This publication has 15 references indexed in Scilit:
- STUDIES ON FLUORESCENT ANTIBODY STAININGThe Journal of Experimental Medicine, 1961
- Chromatographically pure fluorescein and tetramethylrhodamine isothiocyanatesAnalytical Biochemistry, 1961
- Fluorescent protein tracers: the unreacted fluorescent material in fluorescein conjugates and studies of conjugates with other green fluorochromes.1960
- Fluorescent Treponemal Antibody Test. Modification Based on Quantitation (FTA-200)Experimental Biology and Medicine, 1960
- Fluorescent antibody staining of rabies virus antigens in the salivary glands of rabid animals.1959
- Electrophoresis of Fluorescent AntibodyNature, 1958
- Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic with a Modification of its ApplicationExperimental Biology and Medicine, 1958
- ISOTHIOCYANATE COMPOUNDS AS FLUORESCENT LABELING AGENTS FOR IMMUNE SERUM1958
- A Fluorescent Test for Treponemal AntibodiesExperimental Biology and Medicine, 1957
- LOCALIZATION OF ANTIGEN IN TISSUE CELLSThe Journal of Experimental Medicine, 1950