Indirect analysis of corticosteroids. 5. The determination of 17-deoxycorticosteroids
- 1 November 1961
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 81 (2) , 428-434
- https://doi.org/10.1042/bj0810428
Abstract
The Angeli-Rimini reaction was adapted to the determination of small quantities [greater than or equal to]([greater than or equal to]10 [mu]m-moles) of aldehydes. A method was developed for the group determination of 17-deoxycorticosteroids by their conversion into 21-norpregnan-20-als and the measurement of the latter by means of the modified Angeli-Rimini reaction. The method was successfully applied to the determination of 17-deoxycorticosteroids in extracts of acid-treated urines. The above method was modified to permit the separate determination of 21-hydroxypregnan-20-ones and of pregnane-20:21-diols. Applied to urines of normal subjects, the differential assay revealed the presence of substantial quantities of as yet unidentified compounds of the 20:21-diol group.Keywords
This publication has 14 references indexed in Scilit:
- Urinary excretion of C-20 reduction products of corticosterone and 11-dehydrocorticosteroneArchives of Biochemistry and Biophysics, 1960
- The isolation of 16α-hydroxydehydroepiandrosterone (3β:16α-dihydroxyandrost-5-en-17-one) from the urine of normal menBiochemical Journal, 1957
- The isolation of a ketonic dihydroxy Kober chromogen from the urine of pregnant womenBiochemical Journal, 1957
- [Urinary metabolites of corticoids in man; quantitative data].1956
- Indirect analysis of corticosteroids. 4. A note on the role of chloride and urea in the bismuthate treatment of urinesBiochemical Journal, 1956
- Indirect analysis of corticosteroids. 1. The determination of 17-hydroxycorticosteroidsBiochemical Journal, 1955
- THE ISOLATION OF 11-DEHYDR0C0RTIC0STERONE, CORTICOSTERONE AND TETRAHYDRO COMPOUND S (PREGNANE-3α,17α,21-TRIOL-20-ONE) FROM THE URINE OF A MAN RECEIVING CORTICOTROPIN*Journal of Clinical Endocrinology & Metabolism, 1955
- The isolation of pregnane-3α,11β,21-triol-20-one, allopregnane-3α,11β,21-triol-20-one, pregnane-3α,21-diol-11,20-dione and Δ4-pregnene-11β,21-diol-3,20-dione from the urine of normal subjects treated with corticotropinArchives of Biochemistry and Biophysics, 1954
- The oxidation of corticosteroids with sodium bismuthateBiochemical Journal, 1953
- An Investigation into the Determination of Corticosteroids in Urine: I. The Determination of Corticosterone-like SubstancesJournal of Clinical Pathology, 1953