A guide to super-resolution fluorescence microscopy
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Open Access
- 19 July 2010
- journal article
- review article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 190 (2) , 165-175
- https://doi.org/10.1083/jcb.201002018
Abstract
For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new approaches have created unprecedented new possibilities to investigate the structure and function of cells.Keywords
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