A Rapid Radioautographic Technique for Combined Calcified and Soft Tissues

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Abstract
A technic is descr. which enables ground sections (0.3 mm.) to be made from specimens containing soft and hard tissues for purposes of obtaining radioautographs. Fixing and dehydrating is accomplished with dioxan. The embedding is done with a party polymerized plastic by means of a vacuum pump. Final polymerization occurs in 24 hrs. but this time may be reduced to 15 min. for studies with short-lived radioisotopes such as F18. Orientation of the radioautograph is improved by superimposing a roentgenogram on the radioautographic image prior to developing.