Incorporation of hexose nucleoside analogues into oligonucleotides: synthesis, base-pairing properties and enzymatic stability
Open Access
- 25 September 1992
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 20 (18) , 4711-4716
- https://doi.org/10.1093/nar/20.18.4711
Abstract
Oligonucleotides containing 1-(2,4-dideoxy-β-D-erythro-hexo-pyranosyl)thymine (2) and 1-{3,4-dideoxy-β-De-erythro-hexopyranosyl) thymine (3) were synthesized on a solid support using the phosphoramldite approach. The properties of these oligonucleotides were compared with the earlier reported characteristics of oligonucleotides containing 1-(2,3-dideoxy-β-De-erythro-hexopyranosyl) thymine (1). The order in enzymatic stability of end-substituted oligonucleotides is 3>1>>2 . The hybridization properties of the modified oligonucleotides are in reverse order: 2>>1>3.Keywords
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