Loss of a DNA-protein complex correlates with extinguished major histocompatibility complex class II expression in a human B cell.
Open Access
- 1 June 1990
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 171 (6) , 2159-2164
- https://doi.org/10.1084/jem.171.6.2159
Abstract
An E beta DNA protein complex termed complex A, whose binding activity has recently been shown to correlate with both constitutive and regulated class II expression in murine cell lines, is also present in a human B cell, Raji. The DNA involved in complex A, which includes three previously defined transcriptional motifs, W, X, and Y, is a cis-acting transcription element in Raji cells. Both complex A binding activity and transcriptional activity of its target sequence are absent in an Ia- mutant subclone of Raji, RJ 2.2.5. This cell line, whose defect is complemented by a locus on mouse chromosome 16, reexpresses both class II and complex A upon transfection with mouse genomic DNA. We suggest that factors that form complex A or that regulate complex A formation account for the molecular lesion in this cell line.This publication has 11 references indexed in Scilit:
- The presence of a DNA binding complex correlates with E beta class II MHC gene expression.The EMBO Journal, 1990
- Interferon gamma regulates binding of two nuclear protein complexes in a macrophage cell line.Proceedings of the National Academy of Sciences, 1990
- Differences in DNA sequence specificity among MHC class II X box binding proteins.The Journal of Immunology, 1989
- Conserved upstream sequences of human class II major histocompatibility genes enhance expression of class II genes in wild-type but not mutant B-cell lines.Proceedings of the National Academy of Sciences, 1988
- Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box.Proceedings of the National Academy of Sciences, 1988
- A multiplicity of CCAAT box-binding proteinsCell, 1987
- Sequence-specific interactions of nuclear factors with conserved sequences of human class II major histocompatibility complex genes.Proceedings of the National Academy of Sciences, 1987
- Stable integration of mouse DNA into Ia-negative human B-lymphoma cells causes reexpression of the human Ia-positive phenotype.Proceedings of the National Academy of Sciences, 1986
- aIr-1, a newly found locus on mouse chromosome 16 encoding a trans-acting activator factor for MHC class II gene expression.The Journal of Experimental Medicine, 1986
- Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nucleiNucleic Acids Research, 1983