Cell kinetics of normal and perturbed populations measured by incorporation of bromodeoxyuridine and flow cytometry

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Abstract
Bromodeoxyuridine (BrdU) incorporation and flow cytometry have been used to measure the kinetics of V79 cells growing at different temperatures in vitro and cells of the murine Sa F tumor growing in vivo. By simultaneously measuring total DNA content and BrdU incorporation in individual cells at different times after pulse labelling with BrdU, it is a simple procedure to quantify the movement of cells through the cell cycle. The method has the advantage of speed (2 .times. 104 or more cells are analysed within a day or so of the experiment) and the ability to analyse the results in different ways. A G2 block in tumor cell progression in vivo is readily detected after a dose as low as 2 Gy. The neutron relative biological effectiveness for this G2 block is probably larger than that for tumor growth delay.