Identification of Anthocyanins in Berries by Narrow-Bore High-Performance Liquid Chromatography with Electrospray Ionization Detection

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Abstract
Qualitative determination of anthocyanins in extracts of red fruits by narrow-bore HPLC/ESI-MS was carried out. This method was used to investigate anthocyanin contents of black bilberry (Vacciniummyrtillus L.), blackberry (Rubus sp.), and mulberry (Morusnigra). An ultraviolet diode array and a mass spectrometer with ESI source were used for detection. Anthocyanin identifications were made by using retention time data and UV−vis and mass spectra and comparing them with those of commercially available standard compounds. The method allowed the identification of fourteen anthocyanins in black bilberry extract, six anthocyanins in blackberry extract, and five anthocyanins in mulberry extract. Keywords: Anthocyanin; bilberry; blackberry; mulberry; HPLC/ESI-MS; narrow-borecolumn