Survival of Frozen Stored Human and Mouse Bone Marrow Cells

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Abstract
Survival of human bone marrow stored in 15% glycerol (final concentration) in Osgood''s medium was investigated by determination of preservation of morphologic integrity, exclusion of Trypan Blue, and incorporation of tritiated thymidine. The results indicate effective preservation for as long as 7 years. Studied with mouse bone marrow stored for up to 1 year, using the same measurements plus the effectiveness of the marrow in protecting the mice against lethal total-body X-irradiation, suggest that the capacity to incorporate tritiated thymidine correlates most closely with the protective ability of the bone marrow. For prolonged storage, glycerol was found to be more effective than DMSO [dimethyl sulfoxide] as a preservative.