Detection of Platelet Antibody Using Rosette Technique with Anti‐IgG Antibody‐Coated Polyacrylamide Gel

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Abstract
Paraformaldehyde‐fixed platelets from normal donors were used for detection of antibody to platelet byin vitrosensitization (indirect method) utilizing rabbit anti‐human IgG heavy chain specific antibody‐coated polyacrylamide gels (Immunobeads). The sensitized platelets formed rosettes with Immunobeads and the positive rosette count was over 30%, while control showed less than 8% when normal sera were used. This method was also applicable for detecting antibody‐sensitized platelets in vivo (direct method) in patients with autoimmune thrombocytopenia. This method is simple, rapid and reproducible for clinical use. Direct and indirect immunofluorescent antibody tests and a blocking test with anti‐human serum also supported the results of Immunobead rosetting technique.