Three-dimensional tracking of individual quantum dots
- 26 November 2007
- journal article
- Published by AIP Publishing in Applied Physics Letters
- Vol. 91 (22) , 224106
- https://doi.org/10.1063/1.2819074
Abstract
We describe an instrument that extends the state of the art in a single-molecule tracking technology, allowing extended observations of single fluorophores and fluorescently labeled proteins as they undergo directed and diffusive transport in three dimensions. We demonstrate three-dimensional tracking of individual quantum dots undergoing diffusion for durations of over a second at velocities comparable to those of intracellular signaling processes.Keywords
This publication has 21 references indexed in Scilit:
- Confocal three dimensional tracking of a single nanoparticle with concurrent spectroscopic readoutsApplied Physics Letters, 2006
- Reaching out for signalsThe Journal of cell biology, 2005
- Localization Accuracy in Single-Molecule MicroscopyBiophysical Journal, 2004
- Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot TrackingScience, 2003
- Scanning FCS, a novel method for three-dimensional particle trackingBiochemical Society Transactions, 2003
- Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm LocalizationScience, 2003
- Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) GelsScience, 1996
- Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle positionBiophysical Journal, 1994
- Direct observation of kinesin stepping by optical trapping interferometryNature, 1993
- Fluorescent low density lipoprotein for observation of dynamics of individual receptor complexes on cultured human fibroblasts.The Journal of cell biology, 1981