Interferon inhibits transformation of mouse cells by exogenous cellular or viral genes
- 1 June 1983
- journal article
- Published by Springer Nature in Nature
- Vol. 303 (5916) , 433-435
- https://doi.org/10.1038/303433a0
Abstract
Specific genes can be introduced into cultured mammalian cells by DNA transfer, sometimes to be stably integrated and expressed. Because interferon inhibits the transformation initiated by DNA viruses, we wondered if it might also affect transformation induced by recombinant plasmids and cellular genes. We show here that in mouse L cells interferon treatment prevents stable integration and expression of the transfected plasmids containing the cloned herpesvirus thymidine kinase (tk) gene, or the dihydrofolate reductase (dhfr) gene from hamster ovary cells. In contrast, interferon does not prevent transient expression of the tk gene in unintegrated form.Keywords
This publication has 17 references indexed in Scilit:
- High-frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells by protoplast fusion.Molecular and Cellular Biology, 1981
- Altering Genotype and Phenotype by DNA-Mediated Gene TransferScience, 1980
- Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.Proceedings of the National Academy of Sciences, 1979
- DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.Proceedings of the National Academy of Sciences, 1979
- Transfer of purified herpes virus thymidine kinase gene to cultured mouse cellsCell, 1977
- Isolation and partial characterization of three methotrexate-resistant phenotypes from Chinese hamster ovary cellsSomatic Cell and Molecular Genetics, 1976
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973
- Effect of Interferon on Some Aspects of Transformation by Polyoma VirusNature New Biology, 1971
- Cell growth and the initiation of transformation by SV40.Proceedings of the National Academy of Sciences, 1966
- The role of interferon in the inhibition of SV40 transformation of mouse cell line 3T3.Proceedings of the National Academy of Sciences, 1965