Rapid estimation of glycyrrhizin in pharmaceutical preparations by high-speed liquid chromatography.

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Abstract
The method quantitated glycyrrhizin (GA) rapidly, simply and accurately by high-speed liquid chromatography. GA is the active ingredient of glycyrrhiza contained in kanpo (Chinese medical) preparations (tablet, granule, subtle granule, powder or liquid forms), which are made of glycyrrhiza with other crude drugs. Glycyrrhizin contained in such preparations was separated in less than 11 min in DuPont LC 830 with a Permaphase AAX column (50 cm .times. 2.1 mm, i.d. (inner diameter)) by gradient elution technique with 1/30 M phosphate buffer (pH 5.2) and 0.3 M NaClO4 solution as the eluants. Quantitative determination was made by this method of glycyrrhizin contained in 27 different formulas with 6 forms of preparation. For tablet form, a relatively satisfactory correlation was found between the glycyrrhiza extract specified in the prescription and the glycyrrhizin content actually determined. The per cent glycyrrhizin content of preparations containing glycyrrhiza powder (2 granule and 1 powder preparation) was almost identical with the value calculated from the per cent GA content (5%) of glycyrrhiza powder referred to in a previous report.