HORMONAL FACTORS PRODUCING THE GAMETOKINETIC RESPONSE IN THE MALE FROG (RANA' PIPIENS)*

Publisher Website
Google Scholar
Abstract
Various commercial hormone prepns. and FSH 147HM3 (McShan) were injected into the male frog, R. pipiens, and into the immature female Sprague-Dawley rat in order to determine the approx. minimal dose necessary to cause gametokinesis and ovarian hyperemia, respectively. A much smaller quantity of the commercial hormone extracts was needed to evoke rat ovarian hyperemia than to induce gametokinesis, except in the case of pregnant mare''s serum gonadotrophin. Purified FSH 147HM3 caused gametokinesis but 10 mg. of the material failed to produce rat ovarian hyperemia. The rat ovarian hyperemia response appears to be due to the LH factor and to be augmented by the FSH factor. The gametokinetic response in the male frog, R. pipiens, seems to depend primarily on the FSH factor rather than on the LH factor. Urine and/or serum from patients with various clinical syndromes failed to produce the gametokinetic response in the male frog except in the case of 1 patient with myxedema who had received an infusion of 1.5 mg. of epinephrine. Prostigmin, histamine phosphate, dexedrine, neosynephrine, and ACTH (Armour) failed to produce gametokinesis in the dose employed. This comparative study was conducted during the winter and spring months. Preliminary observations indicate that the male frog, R. pipiens, is less sensitive to chorionic gonadotrophin during the summer.