Hemadsorption (Adsorption-Hemagglutination) Test for Viral Agents in Tissue Culture with Special Reference to Influenza

Publisher Website
Google Scholar
Abstract
The phenomenon of hemadsorption is dependent upon selective attachment of erythrocytes onto the monolayer surface of tissue culture cells. It is demonstrated by addition of erythrocytes to a tissue culture system in which propagation of a hemagglutinin-producing virus has occurred. This reaction was used as a means of virus detection in isolation, titration, and identification of influenza A, mumps, and CA (croup-associated) viruses. Prototype strains of 2 other myxo -viruses (Sendai and Newcastle disease virus) and vaccinia produced hemadsorption in appropriate cell cultures. Prototype strains of common non-hemagglutinating viruses (herpes, Coxsackie, ECHO adeno-and polio-viruses) gave negative results. Under some conditions hemagglutinating simian agents, which may be encountered in monkey kidney cell cultures, caused adherence of erythrocytes to the tissue cell sheet, but the characteristic patterns seen with myxoviruses were not observed. Hemadsorption was shown to be a simple, direct, and reliable test offering a new approach to diagnosis of some viral infections. It may also provide an investigative tool for research on virus-cell relationships.