Electron Microscopic Observations on the H Antigen Sites of Human Erythrocytes Using Ferritin Antibody Conjugates

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Abstract
Using immunoelectron microscopy, the distribution of the H antigen sites on human erythrocytes was observed in 40 samples of adult, newborn and fetal blood of different ABO phenotypes. The attached ferritin particles indicating the H antigen sites conspicuously varied in number from cell to cell in every specimen. The number of H antigen sites per single red cell was estimated on an average for each sample as follows: O, 3 .times. 105; B, 2 .times. 105; A1, 1.5 .times. 105; A1B, 105; A2B, 1.5 .times. 105; Ax, 2.5 .times. 105; AxB, 105; Bm, 4 .times. 105; Bw(leukemia), 4 .times. 105; O(newborn), 2.5 .times. 105; B(newborn), 3 .times. 105; A1(newborn), 1.5 .times. 105; A1B(newborn), 2 .times. 105; A1B(fetus), 105. The cells in each sample were divided into 6 cell-populations according to the number of H antigen sites present. The ratios of distribution of such cell populations are compared for all samples.