INVITRO CARCINOGENESIS OF HEPATOCYTES OBTAINED FROM ACETYLAMINOFLUORENE-TREATED RAT-LIVER AND PROMOTION OF THEIR GROWTH BY PHENOBARBITAL

  • 1 January 1980
    • journal article
    • research article
    • Vol. 71  (6) , 747-754
Abstract
The hepatic cells of rats being fed acetylaminofluorene were transferred into culture at various times. Proliferative hepatocytic foci were obtained from animals treated with the carcinogen for > 9 wk. These hepatocytic foci became transplantable and capable of growth in soft agar after 4-12 mo. in culture. Phenobarbital markedly enhanced the growth of these hepatocytes in culture.

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