Improved yields of daunomycinone glycosides in developmental mutants ofStreptomyces coeruleorubidus

Publisher Website
Google Scholar
Abstract
When improvingStreptomyces coeruleorubidus JA 10092, a producer of antibiotics of the daunomycinone complex, the most active variants were found among isolates of morphological typesbld-1 (with a suppressed production of the aerial mycelium on organic media containing glucose) andwhi (with an asporogenic aerial mycelium on glucose media and with the bald phenotype on media containing starch). Submerged cultures of thewhi mutants produced increased quantities of daunomycinone glycosides in the antibiotic complex, the amount of free anthracyclinones being simultaneously decreased. Thewhi strains differed from the wild type also in higher demands for aeration, concentration of glucose and in an increased production capacity in starch media. The overall antibiotic activity increased more than 40 times after a six-step selection (application of UV light, γ-radiation, nitrous acid and natural spreads) combined with an altered fermentation technology.