Hydroxyapatite High-Performance Liquid Chromatography: Detailed Investigations on Chromatographic Efficiencies for the Case of Plate-Like Crystals
- 1 January 1988
- journal article
- research article
- Published by Taylor & Francis in Separation Science and Technology
- Vol. 23 (1-3) , 193-214
- https://doi.org/10.1080/01496398808057642
Abstract
Earlier, hydroxyapatite (HA) high-performance liquid chromatography HPLC) using plate-like crystals was developed. With this HPLC, behaviors of both elution molarity and standard deviation of chromatographic peak are investigated as functions of (a) sample load, (b) flow rate, (c) total column length, and (d) slope of the molarity gradient of the development buffer; from these data, experimental conditions giving optimal chromatographic efficiencies are explored. On the basis of the present study, previously obtained HPLC data are reconsidered. The present study is compared with the earlier study of classical open HA columns. Finally, the data are analyzed on the basis of the chromatographic theory developed earlier, from which important information is available.Keywords
This publication has 14 references indexed in Scilit:
- High-performance liquid chromatography using spherical aggregates of hydroxyapatite micro-crystals as adsorbentEuropean Journal of Biochemistry, 1986
- Hydroxyapatite high-performance liquid chromatography: column performance for proteinsEuropean Journal of Biochemistry, 1985
- Further Analysis of Poly-L-lysine Chromatogram on Hydroxyapatite ColumnSeparation Science and Technology, 1982
- Gradient Hydroxyapatite Chromatography with Small Sample Loads. IV. Gaussian Expression of the Chromatogram and a Further Consideration on the Resolving Power of the ColumnsSeparation Science and Technology, 1981
- Gradient Hydroxyapatite Chromatography with Small Sample Loads. II. Experimental Analysis and the Resolving Power of the ColumnsSeparation Science and Technology, 1981
- The Major Components of the Mouse and Human GenomesEuropean Journal of Biochemistry, 1981
- Theory of chromatography of rigid molecules on hydroxyapatite columns with small loadsJournal of Chromatography A, 1978
- Theory of chromatography of rigid molecules on hydroxyapatite columns with small loads : I. The case when virtually all molecules are adsorbed on to a single type of crystal site through a single type of adsorption groupJournal of Chromatography A, 1974
- Theory of chromatography of macromolecules with rigid structures on hydroxyapatite columns. I. Static partBiopolymers, 1970
- Investigations on the resolving power of hydroxyapatite columnsBiopolymers, 1970